Erwin Heberle-Bors lab

Pollen development and microspore embryogenesis

Alisher TOURAEV, subgroup leader

Tobacco MAP kinases and signal transduction

Cathal WILSON, subgroup leader

Cell Cycle Control of Higher Plants

Laszlo BÖGRE, subgroup leader

Plastid genome rearrangements in microspore-derived albino plants of wheat

Martin PFOSSER, subgroup leader

Experimental papers

1. BÖGRE L, JONAK C, MINK M, MESKIENE I, TRAAS J, HA DTC, SWOBODA I, PLANK C, WAGNER E, HEBERLE-BORS E, HIRT H (1996) Developmental and cell cycle regulation of alfalfa nucMs1, a plant homolog of the yeast Nsr1 and mammalian nucleolin. The Plant Cell 8:417-428.
2. BÖGRE L, LIGTERINK W, HEBERLE-BORS E, HIRT H (1996) Mechanosensors in plants. Nature 383:489-490
3. BÖGRE L, LIGTERINK W, MESKIENE I, BAKER PJ; HEBERLE-BORS E, HUSKISSON N, HIRT H (1997) Wound signalling of plants: activation of a specific MAP kinase pathway. Plant Cell 9:75-83.
4. BÖGRE L, ZWERGER K, MESKIENE I, BINAROVA P, CSIZMADIA V, PLANCK C, WAGNER E, HIRT H, HEBERLE-BORS (1996) The cdc2Ms kinase is differently regulated in the cytoplasm and in the nucleus. Plant Physiology 113:841-852.
5. DAHL M, MESKIENE I, BÖGRE L, HA DTC, SWOBODA I, HUBMANN R, HIRT H, HEBERLE-BORS E (1995) The D-type alfalfa cyclin gene cycMs4 complements G1 cyclin deficient yeast and is induced in the G1 phase of the cell cycle. The Plant Cell 7: 1847-1857.
6. EINZENBERGER E, ELLER N, HEBERLE-BORS E, VICENTE O (1995) Isolation and expression during pollen development of a tobacco cDNA clone encoding a protein kinase homologous to shaggy/glycogen synthase kinase-3. Biochem Biophys Acta 1260:315-319.
7. GARRIDO D, VICENTE O, HEBERLE-BORS E, RODRIGUEZ-GARCIA MI (1995) Cellular changes during the acquisition of embryogenic potential in isolated pollen of Nicotiana tabacum. Protoplasma 186:220-230.
8. HIRT H, BÖGRE L, MESKIENE I, DAHL M, ZWERGER K, HEBERLE-BORS E (1995) Functional isolation and analysis of plant cell cycle genes in yeast. In Molecular and Cellular Biology of the Plant Cell Cycle (D. Dudits, D. Inzé, D. Francis, eds.) The Netherlands: Kluwer Academic Publishers, in press.
9. ILEK A, SWOBODA I, BREITENEDER H, FOGY I, FERREIRA F, SCHMID E, HEBERLE-BORS E, SCHEINER O, RUMPOLD H, KRAFT D, KOLLER HT, BREITENBACH M (1995). Biological functions, isoforms, and environmental control in the Bet v I gene family. In: Molecular Biology and Immunology of Allergens (KRAFT D, SEHON A, eds) CRC Press Inc, Boca Raton, Fl, USA, pp. 245-271.
10. JONAK C, HEBERLE-BORS E, HIRT H (1995) A cDNA from Medicago sativa encodes a protein homologous to small GTP-binding proteins. Plant Physiol 107:263-264.
11. JONAK C, HEBERLE-BORS E, HIRT H (1995) Inflorescence-specific expression of AtK-1, a novel Arabidopsis thaliana homologue of shaggy/glycogen-synthase kinase-3. Plant Mol Biol 27:217-221.
12. LÖSCHENBERGER F, PFOSSER M, HEBERLE-BORS E (1995) Genetic variability for the frequency of doubled haploid green plants is correlated with the ratio of green to albino plants in wheat (Triticum aestivum L.) microspore-derived plants. J Genet & Breed 49:37-44.
13. MESKIENE I, BÖGRE L, DAHL M, HEBERLE-BORS E, AMMERER G, HIRT H (1995) Cloning of a potential G1 cyclin of alfalfa by providing pheromone resistance to yeast. The Plant Cell 7:759-771.
14. MITTERMANN I, HEBERLE-BORS E, VALENTA R (1996) Molecular characterization of profilin isoforms from tobacco (Nicotiana tabacum) pollen. Sex Plant Reprod 9:133-139.
15. MITTERMANN I, SWOBODA I, PIERSON E, ELLER N, KRAFT D, VALENTA R, HEBERLE-BORS E (1995) cDNA cloning and characterization of profilin from tobacco (Nicotiana tabacum): increased profilin expression during pollen maturation. Plant Molec Biol: 27:137-146.
16. PFOSSER M, AMON A, LAFFERTY J, HEBERLE-BORS E, LELLEY T (1995) The gain or loss of single chromosomes in 6x Triticale is detectable by flow cytometry. J Plant Breed 114:555-557.
17. PFOSSER M, AMON A, LELLEY T, HEBERLE-BORS E (1995) Evaluation of sensitivity of flow cytometry in detecting DNA amount variation using disomic and ditelosomic wheat/rye addition lines. Cytometry 21: 387-393.
18. STÖGER E, FINK C, PFOSSER M, HEBERLE-BORS E (1995) Plant transformation by particle bombardment of embryogenic pollen. Plant Cell Rep 14:273-278.
19. SWOBODA I, HA DTC, HEBERLE-BORS E, VICENTE O (1995) Expression of Bet v1, the major birch pollen allergen, during anther development: an in situ hybridisation study. Protoplasma 187:103-110..
20. SWOBODA I, HOFFMANN-SOMMERGRUBER K, O'RIORDAIN G, SCHEINER O, HEBERLE-BORS E, VICENTE O (1996) Bet v 1 proteins, the major birch pollen allergens and members of a family of conserved pathogenesis-related proteins, are ribonucleases. Physiol Plant 96:433-438.
21. SWOBODA I, JILEK A, FERREIRA F, HOFFMANN-SOMMERGRUBER K, SCHEINER O, KRAFT D, BREITENEDER H, PITTENAUER E, SCHMID E, VICENTE O,
22. HEBERLE-BORS E, AHORN H, BREITENBACH M (1995) Isoforms of Bet v 1, the major birch pollen allergen, analyzed by liquid chromatography, mass spectrometry and cDNA cloning. J Biol Chem 270:2607-2613.
23. SWOBODA I, SCHEINER O, HEBERLE-BORS E, VICENTE O (1995) cDNA cloning and characterization of three birch genes, members of the Bet v 1 gene family of pollen allergens, that encode pathogenesis-related proteins. Plant, Cell and Environment 18:865-874.
24. TOURAEV A, FINK CS, STÖGER E, HEBERLE-BORS E (1995) Pollen selection: a transgenic approach. Proc Natl Acad Sci USA 92:12165-12169.
25. TOURAEV A, ILHAM A, VICENTE O, HEBERLE-BORS E (1996) Stress-induced microspore embryogenesis in tobacco: an optimized system for molecular studies. Plant Cell Reports 15:561-565.
26. TOURAEV A, INDRIANTO A, WRATSCHKO I, VICENTE O, HEBERLE-BORS E (1996) Starvation and heat-shock-induced in vitro microspore embryogenesis in wheat (Triticum aestivum L.). Sex Plant Reprod 9:209-215.
27. TOURAEV A, LEZIN F, HEBERLE-BORS E, VICENTE O (1995) Maintenance of gametophytic development after symmetrical division in tobacco microspore culture. Sex Plant Reprod 8:70-76.
28. TOURAEV A, PFOSSER M, VICENTE O, HEBERLE-BORS E (1996) Stress as the major signal controlling the developmental fate of tobacco microspores: towards a unified model of induction of microspore/pollen embryogenesis. Planta 200:144-152.
29. TOURAEV A, STÖGER E, VORONIN V, HEBERLE-BORS E (1997) Plant male germ line transformation. The Plant Journal 12 (4), 949-956.
30. Touraev, A., Heberle-Bors E. (1997) Microspore embryogenesis and in vitro pollen maturation in tobacco. In: Plant Cell Culture Protocols (Robert Hall, ed.), Humana Press, Totowa, USA, in press.
31. VALENTA R, SWOBODA I, GROTE M, VRTALA S, FERREIRA F, DUCHENE M, HEBERLE-BORS E, KRAFT
32. D, SCHEINER O (1995) Profilin: a novel pan-allergen and actin-binding protein in plants. In: Pollen biotechnology: genes, allergens and development (Mohapatra SS, ed). Chapman and Hall, pp. 269-278.
33. WILSON C, ANGLMAYR R, VICENTE O, HEBERLE-BORS E (1995) Molecular cloning, functional expression in Escherichia coli, and characterization of multiple MAP kinases from tobacco. Eur J Biochem 233, 249-257.
34. WILSON C, HEBERLE-BORS E, VICENTE O (1997) A conserved motif at the carboxy terminus of MAP kinases. J. Mol. Evol. 44:683-685.
35. WILSON C, VORONIN V, TOURAEV A, VICENTE O, HEBERLE-BORS E (1997) A developmentally regulated MAP kinase activated by hydration in tobacco pollen. The Plant Cell 9:2093-2100.
36. WILSON C; PFOSSER M; JONAK C; HIRT H; HEBERLE-BORS E; VICENTE O (1998) Evidence for the activation of a MAP kinase upon phosphate-induced cell cycle re-entry in tobacco cells. Physiol Plant, in press.
37. YLSTRA B, TOURAEV A, BRINKMANN AO, HEBERLE-BORS E, VAN TUNEN AJ (1995) Steroid sex hormones stimulate germination and tube growth of in vitro matured tobacco pollen. Plant Physiol 107:639-643.
38. ZARSKY V, GARRIDO D, ELLER N, TUPY J, VICENTE O, SCHÖFFL F, HEBERLE-BORS E (1995) The expression of a small heat shock gene is activated during induction of tobacco pollen embryogenesis by starvation. Plant, Cell and Environment 18:139-147.

Reviews

1. HEBERLE-BORS E (1995) Transformation via microspore embryogenesis. Rice Biotechnology Quaterly 23:17-18.
2. HEBERLE-BORS E; DE VRIES S (1997) Le développment embryonnaire des végétaux. Biofutur 172, 38-42.
3. TOURAEV A, VICENTE O, HEBERLE-BORS E (1997) Induction of microspore embryogenesis by stress. Trends in Plant Sci. 2:297-302.

Publications in popular science

1. 
2. Heberle-Bors (1998) Wie gefährlich ist Gennahrung? Wien Klin Wochensch 110:37-39.
3. HEBERLE-BORS E (1995) Gentechnik in Bezug auf pflanzliche Wirkstoffe. In: PHARMA EXTRA TIME, Nr. 8, pp 1-2.
4. HEBERLE-BORS E (1996) Erdäpfel bleiben Erdäpfel. Chemie 4/1996, p. 8.
5. HEBERLE-BORS E (1996) Herausforderung Gentechnik. Holzhausen Verlag, Wien, 420 pages.
6. HEBERLE-BORS E (1996) Mit Gentechnik gegen Hunger? Eine Kontroverse. Wirtschaft & Umwelt 3/96, pp. 30-31.
7. HEBERLE-BORS E (1996) Von der Antimatschtomate bis zur Turbokuh. Tagungsmappe der Oberösterreichischen Umweltakademie, "Was ist Gentechnik? Welche Chancen bietet sie uns?", Linz, 9.5.1996.
8. HEBERLE-BORS E (1997) Gentechnik und die Haftungsfrage. Profil, Ausgabe 15, p. 144.
9. HEBERLE-BORS E (1997) Heilen mit Genen. In: PHARMA EXTRA TIME, in press.

E-mail address: [email protected]